Three-Dimensional Matrix Derived from Tissue Decellularized of Bone Scaffold Rats Treated with Mixed HA and GAG on Mesenchymal Stem Cells Behavior

Scaffolds provide a suitable environment for growing living cells in vitro and body. Biodegradable and biochemical properties of scaffolds affect the ability of osteogenic cultured cells. In this study, ECM derived from cancellous bone treated with mixed hydroxyapatite and gelatin was used as a three-dimensional environment for investigating the interactions of mesenchymal stem cells with bone matrix. To remove cells from the cancellous bone, physicochemical methods including snap freeze-thaw and sodium dodecyl sulfate (SDS) as an ionic detergent were used. Then scaffolds were treated with mixed hydroxyapatite and gelatin and mesenchymal stem cells cultured on a 3D matrix on different days in vitro. The removals of the cells, treatment, and induction rate of ossification have been confirmed by histotechniques. This study showed that the co-culture of mesenchymal stem cells and 3D scaffolds might be a suitable model to study cell behavior such as migration and polarity in vitro.