Species Identification and Population Structure of Paracaryum (Boraginaceae) of Iran using SCoT molecular markers

Several of the endemic taxa of the distinctive genus Paracaryum, which are found in the Irano-Turanian phytogeographical area, are of unique passion. 139 bands have been generated from the amplification of genomic DNA using 10 primers, 124 of which were polymorphic (91.22%). The high capacity of SCoT primers to identify polymorphic loci among Paracaryum species was demonstrated by the acquired high average PIC and MI values. A range of 0.688 to 0.93 was estimated for the genetic similarity of 12 collections. The examination of SCoT markers revealed that the species Paracaryum bungei and Paracaryum salsum had the most similarity, while P. cyclhymenium and P. intermedium had the least. The aims of the present study are: 1) can SCoT markers identify Paracaryum species; 2) what is the genetic structure of these taxa in Iraq; and 3) to investigate the species inter-relationship. The current investigation has demonstrated that the species may be identified using SCoT markers.