Submit Your Article CMED MEACR meeting
An official publication of the Middle-Eastern Association for Cancer Research
Clinical Cancer Investigation Journal
ARTICLE
Year: 2021   |   Volume: 10   |   Issue: 6   |   Page: 312-317     View issue

Background: P16/Ki-67 dual immunostaining has been confirmed as a sensitive and specific test for human papillomavirus positive women. In the present study, we evaluated cell blocks (CBs) with p16INK4A/Ki-67 biomarkers to detect high-grade cervical intraepithelial neoplasia (CIN). Materials and Methods: Samples for CB preparation were taken from females with abnormal Pap smears, who also underwent colposcopic guided biopsies, P16INK4A and Ki-67 staining were performed on CBs and tissue biopsies, histopathology with p16INK4A expression was considered the gold standard. Sixty-five specimens were included in the study. Results: The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy (AC) of CB + p16INK4A/Ki-67 in detecting CIN2 when considering only cytology specimens with the low-grade squamous intraepithelial lesion (LSIL) were 86.67%, 100%, 66.67%, 89.66%, and 82.93%, respectively. The sensitivity, specificity, PPV, NPV, and AC of CB + p16INK4A/Ki-67 in detecting CIN2 when considering only cytology specimens with atypical squamous cells of uncertain significance/LSIL were 75%, 85%, 60%, 91.89%, 82.69%, respectively. Rates of positive staining for p16 INK4A/ki-67 were enhanced according to increased pathologic grade and differed statistically between CIN1 and CIN2 as well as squamous cell carcinoma. Conclusion: CB preparation technique with p16INK4A and Ki-67 immunostainings have improved the diagnostic AC of Pap smear in detecting high-grade CIN.


Ghefar Omar, Ashraf Olabi, Fatima Alduihi, Lina Ghabreau
Abstract

Background: P16/Ki-67 dual immunostaining has been confirmed as a sensitive and specific test for human papillomavirus positive women. In the present study, we evaluated cell blocks (CBs) with p16INK4A/Ki-67 biomarkers to detect high-grade cervical intraepithelial neoplasia (CIN). Materials and Methods: Samples for CB preparation were taken from females with abnormal Pap smears, who also underwent colposcopic guided biopsies, P16INK4A and Ki-67 staining were performed on CBs and tissue biopsies, histopathology with p16INK4A expression was considered the gold standard. Sixty-five specimens were included in the study. Results: The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy (AC) of CB + p16INK4A/Ki-67 in detecting CIN2 when considering only cytology specimens with the low-grade squamous intraepithelial lesion (LSIL) were 86.67%, 100%, 66.67%, 89.66%, and 82.93%, respectively. The sensitivity, specificity, PPV, NPV, and AC of CB + p16INK4A/Ki-67 in detecting CIN2 when considering only cytology specimens with atypical squamous cells of uncertain significance/LSIL were 75%, 85%, 60%, 91.89%, 82.69%, respectively. Rates of positive staining for p16 INK4A/ki-67 were enhanced according to increased pathologic grade and differed statistically between CIN1 and CIN2 as well as squamous cell carcinoma. Conclusion: CB preparation technique with p16INK4A and Ki-67 immunostainings have improved the diagnostic AC of Pap smear in detecting high-grade CIN.

Cite this article
Vancouver
Omar G, Olabi A, Alduihi F, Ghabreau L. Role of p16INK4A/Ki-67 dual immunostaining on cell blocks in detecting high-grade cervical intraepithelial lesions. Clin Cancer Investig J. 2021;10(6):312-7. https://doi.org/10.4103/ccij.ccij_42_21
APA
Omar, G., Olabi, A., Alduihi, F., & Ghabreau, L. (2021). Role of p16INK4A/Ki-67 dual immunostaining on cell blocks in detecting high-grade cervical intraepithelial lesions. Clinical Cancer Investigation Journal, 10(6),312-317. https://doi.org/10.4103/ccij.ccij_42_21

Sitemap | What's New Feedback | Copyright and Disclaimer | Privacy Notice
© Clinical Cancer Investigation Journal | Published by Wolters Kluwer - Medknow
Online since 01 December, 2011
Editorial and Ethics Policies
Creative Commons  Open Access Journal No author-side fee  View mobile site 
ISSN: Print -2278-1668, Online - 2278-0513