Molecular Evaluation and Study of Organic Base Changes in GCK Gene Related to Gestational Diabetes

Studies in recent years have shown that gene mutations can cause changes in the function of insulin and its receptors, so this change can cause Diabetes during pregnancy and after it. Unlike monogenic diseases, disease incidence is affected by the mutant allele in one gene locus. In diseases similar to type II diabetes, disease incidence depends on several gene loci with a small to moderate impact. The GCK gene encodes a member of the hexokinase family of proteins. Phosphorylated glucose hexokinase to produce 6-phosphate is the first step in most glucose metabolism pathways. Compared to other forms of hexokinase, this enzyme is not inhibited by its product glucose 6-phosphate but remains active when glucose is abundant and leads to diabetic disorder. Thus, this study investigated the frequency of this polymorphism between 50 pregnant women with gestational Diabetes as a patient group and 10 pregnant women without Diabetes or pre-diabetes as a control group. Genomic DNA was extracted by kit method, and PCR and Sequencing techniques were used to determine the genotype of individuals. The frequency of GG and GA genotypes for GCK mutation (rs1799884) was 8% and 92%, respectively, in the patient group and 100% in the control group.