Effect of platelet extract on proliferation and migration of fibroblast cell

Nowadays, one of the most important cells involved in wound healing are fibroblasts, which play a key role in promoting the healing process. Hence, the present study aims to investigate the effect of different concentrations of platelet extract derived from umbilical cord blood on the proliferation and migration of fibroblast cells. The present study is a case-control study that used mature human fibroblast cells. Platelet extract derived from umbilical cord blood plasma was prepared and then fibroblasts were treated with different concentrations (UCB-PL). The results of the present laboratory have shown that 10% concentration is the highest effective concentration in cell migration. Also, the results of the present study revealed that there was no increase in proliferation with the increase in concentration, meaning that in 20% and 15% concentrations in 3 hours of 24, 48, and 72 hours, a decreasing trend in proliferation was also observed. In the case of the negative control, the obtained results show that this group has a significant difference with all groups except the 20% concentration group (p≤0.05), and in the case of the UCB-PL/FBS group, an increase in proliferation compared to the negative control and positive control groups has been shown in the 3 mentioned times (p≤0.05). The results revealed that platelet extract, derived from umbilical cord blood, can proliferate and migrate the fibroblast cells, which are key functions in the wound healing process.