Contribution of APC gene in rs1255190244 mutations on the colon cancer patients in Tehran Province

Mutations on the adenomatous polyposis coli (APC) gene can cause colon cancer. Colon cancer is a kind of cancer that starts in the large intestine (colon). cancer colon begins with overgrown cells in a body.

Objectives: the point of this studying field is analyzing APC gene in rs1255190244 mutations on the colon cancer patients and in Tehran Province.

Methods: From 1397 to 1398, one hundred samples of 6 to 10 ml per patient were collected from patients with colorectal cancer who were definitively diagnosed. Then the DNA of each sample was extracted and after checking the quality of the DNAs by electrophoresis method, the required primers were designed that all primers were designed at almost the same annealing temperature. After performing all PCR steps, the information was surveying and Conclusions were made.

Results: the results which obtained in this study, it shows that the incidence of rs1255190244 in the patient group compared with the control group with P value = 0.003 was significantly associated with the incidence of colon cancer.

Conclusions: From the results of this study, it can be concluded that the polymorphism rs1255190244 can be used as a marker to prevent colon cancer, but more research is needed to further investigate other polymorphisms..