Comparison of ppe44 + HSPX + FC and PPE44 + HSPX complexes in MTB

One of the challenges in controlling tuberculosis is the many defects in the BCG vaccine. It is necessary to design and produce an effective and safe recombinant complex against this disease that covers the shortcomings of BCG.

  For our study entitled: Comparison of ppe44 + HSPX + FC and PPE44 + HSPX complexes in MTB

  It was extracted from NCBI, PPE44 and HSPX gene and mouse IgG2a Fc.The design was done according to a previous study by the same authors.

Cloning in pet28a and vector transfer in E. coli Bl2, purification and SDS-PAGE were performed. 35 BALB/c mice were divided into 5 groups, injected on days 0 and 21 and blood sampling on days 14 and 45. Total antibodies and IgG1 and IgG2 subclasses were investigated and the production level of cytokines IFN-γ, IL-2 and IL-. 4, TNF-α, and TGF-β.

Findings: A significant correlation was observed in the amount of total antibody in the second stage of the assay at a concentration of 1.20 between the groups receiving PHF and PH. (p<0.05.

There was a significant increase in the amount of IgG1 in the group with PHF and IgG2 in IL+ PHF compared to other groups. (p < 0.05)

There was a significant increase in the level of IFN-γ, TNF-α, TGF-β, IL-2 and IL-4 in PHF (p<0.05).

Conclusion: Recombinant proteins were designed to strengthen the immune system and the addition of IL-22 improved the level of antibody production. Covered one of the most important defects in the common BCG vaccine.