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Clinical Cancer Investigation Journal
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Year: 2020   |   Volume: 9   |   Issue: 4   |   Page: 110-113     View issue

Comparison and frequency of cell cultured in 48-h and 72-h mitotically treated with phytohemagglutinin-M: Both promise for normal and cancer cells


Alireza Abadi, Zeinab Mazloumi, Ali Kolahi, Mojgan Sheikhpour, Mostafa Rezaei-Tavirani, Afshin Moradi, Mahsa Ahadi, Fatemeh Aghamaleki, Niloufar Safavi, Mohammad Heidary, Mahdi Zamani, Sima Rad, Maliheh Entezari, Mehrdad Hashemi, Abolfazl Movafagh
Abstract

The aim of the present study is hence to comprise of mitotic index outcome in the individuals of normal volunteers involved in two different period of culture with effect of Phytohemagglutinin-M. Comparison of healthy human donors lymphocytes were cultured for 48-h and 72-h and using conventional karyotype technique with minor modification. Mitotic index and cell proliferation frequency were analyzed in the individuals of normal blood donors after preparing of colchicine to cultured metaphases 2 h before cell fixation. The metaphase indices at 48-h and 72-h culture period resulted a perfect yield product. Lymphocytes populations in 72-h laboratory work were more than 48-h cultures (2.73% at 72 h vs. 1.48% at 48 h, P ≤ 0.05). The present research indicates significant comparisons for laboratories and individuals to be set up. With effect of these results, it may help in time-consuming and short time period for patient, as well as fresh suggestion for health-care management decision. Reagent, manual, budget, time for diagnosis, and health-care fulfillment may resolve of this proposal.

Cite this article
Vancouver
Abadi A, Mazloumi Z, Kolahi A, Sheikhpour M, Rezaei-Tavirani M, Moradi A, et al. Comparison and frequency of cell cultured in 48-h and 72-h mitotically treated with phytohemagglutinin-M: Both promise for normal and cancer cells. Clin Cancer Investig J. 2020;9(4):110-3. https://doi.org/10.4103/ccij.ccij_6_20
APA
Abadi, A., Mazloumi, Z., Kolahi, A., Sheikhpour, M., Rezaei-Tavirani, M., Moradi, A., & et al (2020). Comparison and frequency of cell cultured in 48-h and 72-h mitotically treated with phytohemagglutinin-M: Both promise for normal and cancer cells. Clinical Cancer Investigation Journal, 9(4),110-113. https://doi.org/10.4103/ccij.ccij_6_20

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