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Cancers, especially of the neural tissue, are often deemed a death sentence. There is, however, still no clear understanding of the underlying causes nor the cellular and molecular mechanisms of this disease. The spread and collateral damage caused by various types of brain tumors remain poorly understood, despite the information that is currently available about these diseases. A common means of diagnosing tumors is through cerebrospinal fluid (CSF) leakage, and the enzymes from brain cancer cases were investigated within this hypothesis. The purpose of this study was to investigate the role of CSF in brain cancer and BBB. This study conducted a systematic study of leakage that typically occurs at the spine level, namely in the thoracic spine region and the base of the brain along the cardiothoracic connection. This study investigated the bacteria sampling of brain cancer in CSF and determined the common method of targeting cancer cells in the brain and enzymes contained within the CSF. A further finding reveals the precise foci of this leakage and various proteins and enzymes that may be responsible for this damage, as well as evidence that the release of tumor components damages the CSF. As a result of these observations, enzymes and tumor cells are detected, and a new component identifies tumor-related CSF.
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