TY  - JOUR
T1  - Role of fibrotic cancer stroma in rectal carcinoma: An immunomorphological assessment
A1  - Navpreet Kaur
A1  - Sufian Zaheer
A1  - Preeti Sharma
A1  - Vaishali Rohilla
A1  - Sunil Ranga
JF  - Clinical Cancer Investigation Journal
JO  - Clin Cancer Investig J
SN  - 2278-0513
Y1  - 2021
VL  - 10
IS  - 6
DO  - 10.4103/ccij.ccij_3_21
SP  - 289
EP  - 293
N2  - 
Background: The aim of the study is to evaluate the role of fibrotic cancer stromal response and tumor budding in ectal adenocarcinoma development and progression. Materials and Methods: Fibrotic cancer stroma was classified into three distinct histological categories, i.e. mature, intermediate, and immature. The number of tumor-budding foci was counted in the low-power field (×10), and 0–5, 5–9 and ≥10 tumor buds were scored as I, II, and III, respectively. All histological and immunohistochemical assessments were made at the invasive front of the tumor. The distribution of T lymphocytes and myofibroblasts was assessed by immunohistochemical reactivity for the cluster of differentiation 3 and anti-smooth muscle antibody actin, respectively. Results: Among 25 cases of rectal carcinoma, 60% (15 cases) of patients had mature fibrotic cancer stroma, whereas 28% (7 cases) of patients had intermediate stroma and 12% (3 cases) of patients had immature stroma. The cancer-specific 5-year survival rate in the groups with mature stroma, intermediate stroma, and immature stroma was 53.34%, 42.8%, and 33.34%, respectively. There was a statistically significant correlation between the category of fibrotic cancer stroma and the tumor budding. Further, on immunohistochemical analysis and counting, the average number of T-cells was 302/400 μm diameter field in the region of mature fibrotic stroma, in comparison with 197/400 μm and 92/400 μm in the intermediate and immature fibrotic stroma, respectively (unpaired t-test with P < 0.05). Myofibroblasts were observed in 20% of tumors with mature fibrotic stroma compared with 65% in the intermediate fibrotic stroma and 100% of the tumors with immature fibrotic cancer stroma. Conclusions: The histological classification of fibrotic cancer stroma highlights the role of the stromal response with respect to host immune reaction and behavior in rectal adenocarcinoma and acts as a useful tool for predicting patient prognosis and outcome.

UR  - https://ccij-online.org/article/role-of-fibrotic-cancer-stroma-in-rectal-carcinoma:-an-immunomorphological-assessment-932
ER  -