%0 Journal Article
%T Reduction in the numbers of CD33+ myeloid population in Egyptian children with B-linage acute lymphoblastic leukemia and its recovery after induction of chemotherapy
%A Mohamed Salem
%A Mohamed El-Shanshory
%A Randa El-Naggar
%A Said Abdou
%A Mohamed Attia
%A Abdel-Aziz Zidan
%A Mona Zidan
%J Clinical Cancer Investigation Journal
%@ 2278-0513
%D 2015
%V 4
%N 5
%R 10.4103/2278-0513.164716
%P 627-632
%X Background:  Acute lymphoblastic leukemia (ALL) is biologically and clinically considered as a heterogeneous neoplasm of lymphoid progenitor cells. About 85% of children with ALL are diagnosed as B-ALL, expressing CD19; the typical marker of normal B cells. Problem: Given that the chemotherapy associated with leucopenia, in particular myeloid cells (CD33+ cells), Aim: the main aim of this study was to analyze the numbers of these cells in children with B-ALL before and after induction of chemotherapy. Materials and Methods:  The frequencies of CD33+ myeloid cells and CD19+ B-cells were analyzed in the peripheral blood patients before (n = 10) and after (n = 10) induction of chemotherapy as well as in healthy volunteers (n = 10) using multiparametric flow cytometry. Results:  As expected, B-ALL patients showed high numbers of CD19+ cells before induction of chemotherapy; where the numbers of these cells were reduced upon the induction of chemotherapy. CD33+ myeloid cells showed decrease in numbers in B-ALL patients before chemotherapy as compared to healthy control volunteers. Interestingly, treatment of B-ALL patients with chemotherapy-induced almost recovery of the numbers of these cells. Conclusion:  CD33+ myeloid cells are increased in numbers after induction of chemotherapy, indicating to a dynamic mobilization or differentiation of their precursors into circulation. This study opens a new avenue to characterize the phenotype and function of these cells in different hematological malignancies; in particular, they may harbor regulatory cells.
%U https://ccij-online.org/article/reduction-in-the-numbers-of-cd33+-myeloid-population-in-egyptian-children-with-b-linage-acute-lymphoblastic-leukemia-and-its-recovery-after-induction-of-chemotherapy-470