TY  - JOUR
T1  - Children with acute lymphoblastic leukemia show high numbers of CD4+ and CD8+ T-cells which are reduced by conventional chemotherapy
A1  - Mohamed Salem
A1  - Mohamed El-Shanshory
A1  - Nabila El-Desouki
A1  - Said Abdou
A1  - Mohamed Attia
A1  - Abdel-Aziz Zidan
A1  - Shymaa Mourad
JF  - Clinical Cancer Investigation Journal
JO  - Clin Cancer Investig J
SN  - 2278-0513
Y1  - 2015
VL  - 4
IS  - 5
DO  - 10.4103/2278-0513.164717
SP  - 603
EP  - 609
N2  - Background: Acute lymphoblastic leukemia (ALL) is considered as one of the most common cancer in pediatric malignancies. Among ALL, B-cell Acute Lymphoblastic Leukemia (B-ALL) represents 80% to 85% of the childhood ALL. 
Problem: Although anti B-ALL chemotherapy kill B-ALL, it associates with alteration in the numbers of CD4+ and CD8+ T-cells, and thus impacts the overall immunity. 
Aim: To evaluate the impact of anti B-ALL on the numbers of CD4+ and CD8+ T-cells in correlation to the numbers of CD10+ B cells in B-ALL pediatric patients. 
Materials and Methods: Peripheral blood samples were drawn from previously diagnosed B-ALL before (n = 10 cases) and after (n = 10 cases) chemotherapy as well as from healthy controls (n = 10 cases). The numbers of CD4+, CD8+ T-cells and CD10+ B cells were measured in these samples by flow cytometry. 
Results: As expected, the numbers of CD10+ B-cells were increased in B-ALL patients before chemotherapy which were associated with increases in the numbers of CD4+ and CD8+ T-cells. Chemotherapy of B-ALL patients, during the induction phase, induced dramatic decreases in the numbers of CD10+ B cells, which were associated with decreases in the numbers of CD4+ and CD8+ T-cells. Tin spite of this alteration, the ratio of CD4/CD8 in B-ALL patients were remained similar before and after chemotherapy as compared to those in healthy controls. 
Conclusion: Anti B-ALL chemotherapy induces alterations in the frequencies of T-cell subsets. Given the importance of these cells in anti-tumor immunity, our data may lead to further studies to investigate the different subsets of these cells, in particular regulatory T-cells.
UR  - https://ccij-online.org/article/children-with-acute-lymphoblastic-leukemia-show-high-numbers-of-cd4+-and-cd8+-t-cells-which-are-reduced-by-conventional-chemotherapy-466
ER  -